Journal: The FASEB Journal

Article Title: Resveratrol‐enhanced SIRT1‐mediated osteogenesis in porous endplates attenuates low back pain and anxiety behaviors

doi: 10.1096/fj.202002524r

Figure Lengend Snippet: FIGURE 2 Resveratrol attenuates inflammation and sensory innervation in endplates. A-C, ELISA analysis of the PGE2, IL-1β, and RT- PCR analysis of expression of TNFα in the lumbar endplates lysates from mice in the sham, vehicle and 40 mg/kg/d resveratrol treated groups at 4 and 8 weeks post-LSI surgery. D-E, Representative images of the immunofluorescence and quantitative analysis of COX2+ cells (green) in endplates from mice in the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar: 100 μm. F-G, Representative images of the immunofluorescence and quantitative analysis of CGRP+ sensory fibers (green) in the endplates from mice in the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar: 50 μm. H-I, Representative images of the immunofluorescence and quantitative analysis of the CGRP+ sensory neurons (green) in L2-4 DRGs from mice in the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar: 100 μm. N ≥ 6 per group. *P < .05, **P < .01. (Student’s t test)

Article Snippet: The concentrations of PGE2 and IL- 1β in the L4- 5 endplates and the concentration of BDNF in the hippocampus were measured by a PGE2 ELISA kit (514010, Cayman Chemical, Ann Arbor, MI, USA), IL- 1β ELISA kit (BMS6002, Thermo Fisher, Waltham, MA, USA), and BDNF ELISA kit (dy248, R&D System, Minneapolis, MN), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunofluorescence

Journal: The FASEB Journal

Article Title: Resveratrol‐enhanced SIRT1‐mediated osteogenesis in porous endplates attenuates low back pain and anxiety behaviors

doi: 10.1096/fj.202002524r

Figure Lengend Snippet: FIGURE 4 Resveratrol promotes osteogenesis in the porous endplates after LSI surgery. A-B, Representative immunofluorescence images of the Osterix staining in the L4-5 endplates and the quantitative analysis of the number of osterix+ cells per section from the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar, 20 μm. C-D, Representative images of the calcein double-labeling of porous endplates with quantification of the mineral apposition rate from the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery, distance between white arrowheads were measured. E-F, Representative images of Trap staining of the endplates and quantitative analysis of the osteoclast number per section from the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar, 50 μm. G-H, The RT-PCR analysis of Slit3 and Runx2 expression in the L4-5 endplates from the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. I, The quantitative ELISA evaluation of Netrin-1 concentration in the L4-5 endplates from the sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. N ≥ 6 per group. *P < .05, **P < .01. (Student’s t test)

Article Snippet: The concentrations of PGE2 and IL- 1β in the L4- 5 endplates and the concentration of BDNF in the hippocampus were measured by a PGE2 ELISA kit (514010, Cayman Chemical, Ann Arbor, MI, USA), IL- 1β ELISA kit (BMS6002, Thermo Fisher, Waltham, MA, USA), and BDNF ELISA kit (dy248, R&D System, Minneapolis, MN), respectively.

Techniques: Immunofluorescence, Staining, Labeling, Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: The FASEB Journal

Article Title: Resveratrol‐enhanced SIRT1‐mediated osteogenesis in porous endplates attenuates low back pain and anxiety behaviors

doi: 10.1096/fj.202002524r

Figure Lengend Snippet: FIGURE 7 Resveratrol alleviates hippocampal inflammation and enhances pCREB activation, BDNF expression in the hippocampus. A-C, The RT-PCR analysis of the expression of the inflammatory mediators TNFα (A), IL-1β (B), and IL-6 (C) in the hippocampus of resveratrol- treated and control mice at 8 weeks post-LSI surgery. D, Representative images of the pCREB immunostaining at the dentate gyrus of the hippocampus from sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar, 200 μm. E-F, Representative images of the BDNF immunostaining at the dentate gyrus of the hippocampus and the quantitative ELISA analysis of BDNF in the hippocampus from sham, vehicle, and resveratrol groups at 8 weeks post-LSI surgery. Scale bar, 200 μm. *P < .05. (Student’s t test)

Article Snippet: The concentrations of PGE2 and IL- 1β in the L4- 5 endplates and the concentration of BDNF in the hippocampus were measured by a PGE2 ELISA kit (514010, Cayman Chemical, Ann Arbor, MI, USA), IL- 1β ELISA kit (BMS6002, Thermo Fisher, Waltham, MA, USA), and BDNF ELISA kit (dy248, R&D System, Minneapolis, MN), respectively.

Techniques: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Immunostaining, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing